Factors That Affect Enzyme Reaction Rate

Lab Report Factors That Affect Enzymes Reaction Rate Name of lab: Effects of temperature, pH, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity Introduction: Enzymes are the most important types of proteins, they act as catalysis (speed up chemical reactions). If enzymes didn’t exist, biochemical reactions would act to slowly and they couldn’t keep up with the metabolic functions. Enzymes have a three-dimensional structure that is really complex. This structure consists of one or more polypeptide chains, they form an active site, which is an area in which the substrate eventually will fit.The four factors that affect the activity and reaction rate of an enzyme are temperature, pH, enzyme concentration and substrate concentration. Research Question/Hypothesis: The hypothesis is that when the temperature is higher than 40? C, the enzyme catalysis will increase. That the enzyme activity with the pH effect is that if it isn’t at the optimum pH v alue there will be a total loss of the enzyme activity. As the change in enzyme concentration increases the activity rate will increase if there is a proportional amount of enzyme concentration and substrate.Variables: Independent: pH, enzyme concentration, substrate concentration and enzymatic activity. Dependent: the reaction rate Control variable: temperature and amount of substrates and enzymes added. Materials: Phosphate Buffers Beaker Catechol Potato Juice Parafilm Test Tubes Procedure: To study the effect of temperature: 1. Three different test tubes where filled with 3mL of phosphate. 2. They were set in three different temperature settings. First tube was placed in an ice-water bath for ten minutes, the second in a room temperature until 21?C was reached, and the third tube was placed in a beaker of warm water. To study the effect of pH: 1. The pH values of pH 4, pH 6, pH 7, pH 8, and pH 10 was used to fill five separate test tubes with 3 mL each. 2. To each test tube, ten drops of catechol and potato juice were added, each tube was covered with Parafilm, and inverted several times to mix the contents. After letting each tube stand for three to five minutes the tubes were inverted at one minute intervals. To study the effect of enzyme concentration: 1.The steps include four test tubes that were labeled A, B, C, and D. 2. The contents added to tube A are 3mL plus 20 drops of pH 7 phosphate buffer, but no potato juice. To tube B, 3 mL plus 15 drops of pH 7 phosphate buffer and 15 drops of potato juice. The amount of pH 7 phosphate buffer added to test tube C are 3 mL plus 10 drops of potato juice. To test tube D, 3 mL of pH 7 phosphate buffer and 20 drops of potato juice were added. 3. Then, each tube was covered in Parafilm and inverted several times to mix the contents.After doing so, 10 drops of catechol were added to each tube and covered with Parafilm and inverted several times to mix the contents. 4. After allowing each tube to stand for three to four minutes the tubes were mixed at one-minute intervals. To study the effects of substrate concentration: 1. Eight test tubes that were involved. 2. These test tubes were labeled 1, 2, 4, 8, 16, 24, 32, and 48. Next, each test tube had a certain amount of phosphate buffer and catechol was added to each. 3.To test tube #1: 5 mL plus 47 drops of pH 7 phosphate buffer and 1 drop of catechol; test tube #2: 5 mL plus 46 drops of pH 7 phosphate buffer and 3 drops of catechol; test tube #4: 5 mL plus 44 drops of pH 7 phosphate buffer and 4 drops of catechol; test tube #8: 5 mL plus 40 drops of pH 7 phosphate buffer and 8 drops of catechol; test tube #16: 5 mL plus 32 drops of pH 7 phosphate buffer and 16 drops of catechol; test tube #24: 5 mL plus 24 drops of pH 7 phosphate buffer and 24 drops of catechol; test tube #32: 5 Ml plus 16 drops of pH 7 phosphate buffer and 32 drops of catechol; and test tube #48: 5 mL of pH 7 phosphate buffer and 48 drops of catechol. 4. Then, each tube was c overed with Parafilm and inverted several times to mix the contents. Also, to each of the tubes, 30 drops of potato juice were added and covered with Parafilm and inverted several times to mix the contents. The test tubes set at room temperature for five minutes and were mixed at one minute intervals.

Women in Sport Case Study Example | Topics and Well Written Essays - 1500 words

Women in Sport - Case Study Example Before 1870, women sports subsisted in â€Å"play activity: forms that were entertaining instead for competition, also, being casual and lacking regulations, highlighted physical exercise (Shangold, Mona & Gabe, 2012). A leading belief in 1800 was that every person had a predetermined energy amount. If such energy gets employed in physical as well as academic duties simultaneously, it may be dangerous (Women's Sports Foundation, 2006). Riding on the back of a horse for recreation, show canoeing as well as swimming became trendy; however women weren’t motivated to dive themselves. This bodily exercise for women was considered to be particularly dangerous since in menstruation they were â€Å"occasionally weakened† (Adrian, 2005). During 1875, while women were starting to get entry to universities, Prof. Edward Clarim released Gender in School; or, A Just Chance for Girls that ignited an insistent and bitter discussion concerning the women’s capability for bodily exercise. He articulated that, â€Å"every muscular as well as mind labor may be decreased at the menstruation onset† (Parkhouse & Lapin, 2015). While extra women pursued to get included in sports, they develop into being further competitive. During the belatedly 1800 as well as early 1900, they started to create unofficial athletic associations. Bowling, archery, croquet and Tennis were trendy in associations from in New Orleans and New York.

Introduction to film 2 Essay Example | Topics and Well Written Essays - 1000 words

Introduction to film 2 - Essay Example Compared with other media, the distinctness of film is verisimilitude (Metz, quoted in Lechte 1994: 78) – creating a definite kind of credible resemblance or similarity to the real world (Kuhn 1985: 269). Unlike theatre, which is too real, film obtains its real power from its ability to produce reality by crossing unreality. Ironically, it is on this basis – creating an illusion of reality – that film achieves its realism, because it is this capacity of film, that the attitude of the spectator of ‘suspension of disbelief’ is achieved and where the power of film depends (Lechte 1994: 78). This â€Å"experience of realism in cinema is intricately tied to the impression, that the events and persons depicted are somehow real† (Riis 2002: 93). Here also lies the paradox of films: despite the knowledge that films are only screened, spectators still genuinely felt the portrayed events are genuinely happening (Riis 2002: 93; Kuhn 1985: 268). Also, thi s ‘impression of reality’ – the film’s defining mark, gives film the privilege to challenge analysis (Lechte 1994: 78). Films usually use three different styles in screenwriting: narrative/linear – tells the story chronologically, non-linear – defies chronology for example Quentin Tarantino’s â€Å"Pulp Fiction† (1994) and documentary – presenting reality in real-time (Niche Express.net 2009: 1-2). Fiction usually utilises linear and non-linear, while documentary film the documentary. Although realism principally characterises both fiction and documentary film (Kuhn 1985: 269), they still differ in their notion of representation, verisimilitude, realism, and reality, distinguishing them from each other. Documentary films are â€Å"strictly speaking †¦ non-fictional, ‘slice of life’ factual works of art †¦ [also] known as cinema verite† (Dirks 2009: 1) – in French means ‘cinema truth’ (Niche

(Consumer behavior) Critically discuss the barriers associated with Essay

(Consumer behavior) Critically discuss the barriers associated with perceived risk in decision-making processes for the chosen - Essay Example The company may instigate customer interest by creating a website which maps the development of the product as Toyota had done before the launch of Prius. Also creating online brochures and e-marketing are ways to reach more customers. Discussion The barriers associated with perceived risk in decision-making processes Petrol-electric hybrid cars are launched as alternatives to conventional, internal combustion engine cars. The hybrid automobiles are aimed at achieving higher economy of fuel by combining a conventional engine with a rechargeable battery. The fuel economy of hybrid cars are important in today’s world in perspective to the climatic changes as well is because of the concerns of energy security. The use of petrol-electric hybrid cars causes almost half of the carbon dioxide emissions petrol consumed in conventional fuel cars. Hybrid cars are capturing an increasing share of the domestic automobile (The New York Times, 2007, p.2). Though hybrid cars are not widely i ntegrated into the market still it is a growing range of product and can be successfully used to combat energy and ecological issues. Therefore, it is vital to know the main influencing factors which influence the decision making process of a customer in buying a hybrid car. Some factors are similar to those arising in the acceptance of a new technology by the consumer market. In consumer behaviour, the choice of a product depends on a varying number of factors. In the case of choosing an automobile for buying, customer decision making is a high involvement and often quite complicated process. An automobile, though being a product for regular use, it comes under a product category which is rarely bought and bought after an intricate evaluation process followed by the customer. Also, a car is expensive, a certain range of automobiles come under the premium products category and a number of alternative brands and types can be found in the market. Additionally, the customer has the per ceived risk of not choosing the perfect automobile to suit his needs and having to bear a huge cost to rectify the mistake and cars being an investment for a long time, consumers show the trend of having a more complex and long process of decision making. The consumer decision making process involves both intrinsic and extrinsic methods (The Wall Street Journal, 2009). Mostly, hybrid cars are seen to be purchased for social reasons and concern for environment is a lesser influential factor in the consumer decision making process. It is seen that though many people are aware of the potential damage and threats to then ecological system and want to help in balancing the environment, the willingness of the customers rarely changes into the actual buying behaviour due to the above mentioned factors that majorly influence their buying behaviour. The increase in energy prices and incomes largely and most significantly impact the buying behaviour of the consumers for the hybrid vehicles (E dwards, 2010, p. 56). Some factors like high energy consumption and

Jury Research Paper Example | Topics and Well Written Essays - 750 words

Jury - Research Paper Example Justification of the reforms to a state legislature There have been various reforms initiated with the objective of promoting racial diversity in the composition of juries, so that effective decisions and proper, unbiased judgments can be made in the courtroom. However, the construction of the jurisdiction is also not without the criticism of racial diversity. In the American society, the minority class people are often allegedly ascertained to be treated in a bias manner, which further restrict justice on the basis of their rights and privileges as a US citizen. In this respect, the alleged discrimination amid minority and majority people has raised various ethical issues which include assaults and movements against the State (Minow, 1992). There are instances where the representatives of the juries have been witnessed to evolve their judgment with elusive goals. This raises significant concern with regard to the rationality of the judgment offered in the courtroom, where jurors bel onging to the same race as that of the defendant have been alleged to act with sympathy; while on the other hand, jurors belonging to other races tend to implement harsh treatments for punishing the convicts. Hence, it can be argued that the failures of appropriate jurisdiction processes are caused mainly due to lack of diversity in the configuration of jurors. Thus, maintaining a higher degree of racial diversity amid juries is most likely to contribute towards making effective and racially unbiased judgment in the courtroom. Subsequently, with the involvement of minorities and women as jurors, the judgment process may also obtain an enhanced efficiency in dealing with equality of rights and mitigating biasness to a substantial extent (SAGE Publications, 2013). An opponent’s response to the reform There is need for racial diversity in the jury system so that effective decisions can be made with equality and in an unbiased manner. However, from an opponent’s point of v iew, with majorities and minorities, as representatives of the jury, the defendants must not to be offered with the opportunity of selecting as well as composing juries in accordance with their races and communal backgrounds. As in the case of Swain v. Alabama - 380 U.S. 202 (1965) (Justia, 1965) and Batson v. Kentucky (No. 84-6263) (LII, 1985), the judiciary composed in the trials of these cases included majority of jurors belonging from the petitioners respective racial backgrounds and communities, which led to biasness in the process decision making. The defendants, with this facility of composing juries on the basis of their choices, will certainly lead to inappropriate legal decisions and procedures. In this concern, the provisions stipulated by the Supreme Court of the US should firmly prohibit defendants from allocating rights of selecting jury members for the execution of judgment with equality and appropriateness. Furthermore, it stresses that the juries should be selected on the basis of merit system amid majority and minority group of individuals, so that the decisions or judgment are

Vaccines and Aspergers Syndrome Research Paper Example | Topics and Well Written Essays - 750 words

Vaccines and Aspergers Syndrome - Research Paper Example People who have the Asperger’s Syndrome show recurring and cyclical behavior through their actions and manners, physical gawkiness and clumsiness and display of passionate and extreme interest in a particular thing. The precise and exact cause is not yet known and is unidentified although several factors and aspects might cause it. One cause is certain and that is genetics. For sure, genetics have played a big role in the world of autism spectrum. Some other possible causes are due to the environment. One of the theorized and said causes of Asperger’s Syndrome is vaccination. Vaccines are very helpful and have been greatly beneficial and useful to the community and to the society. It has greatly helped the general public by providing agents that would bear a resemblance to a weak or killed disease-causing and pathogenic micro-organism that is injected in to the body to provide immunity. It stimulates and incites the system of the body to remember and destroy the foreign organism. Although it has been a great help to the world, it might also have its side effects that can cause autism to the future generations to come. People have been continuously arguing and debating over the subject on how vaccines might be linked to Asperger’s Syndrome. One of the main matters talked about is thimerosal, which is a preservative in vaccine and is also used in tattoo inks and certain products.

Planning for the Future Essay Example for Free

Planning for the Future Essay Hopes and Dreams A. Even if you have not done much research on the topic what are your hopes and dreams for your employment future? â€Å"What do you want to be when you grow up? † -When I grow up I want to be an ultrasound technician. I’m very interested in the female body and reproductive system. I also love babies so it was keep me interested in my studies. I want to be able to help women out with the beautiful experience of birth and child development. B. What do you want your life to look like in 10 years? In 10 years I want to be a successful ultrasound technician. I will also make my own schedule so I can spend time with my amazing future family. I want to be living by the beach with my husband, Jason Zeigler, and my two children, Carson and Brayden. I will be in the upper-class and have a river house. We will have two dogs, Jackson and Parker. C. What do you want your life look like in 5 years? -In 5 years I will be in college. Hopefully University of Riverside after I get my general education done at Chaffey. I will also be moved in and engaged to Jason Zeigler. That will be the biggest turning point of my life in my own personal opinion mainly because I will be focusing on my major and my career, which will in turn determine the rest of my life. Either successful or a failure. D. What are you doing now that will affect the rest of your life? -Things that I am doing now that will affect the rest of my life is graduating high school, going to college, and working and becoming independent. Graduating is a huge step in life. It’s the start of the rest of our lives. There’s no more messing around anymore. We are growing up and now we must be independent and do things on our own. Going to college is another big step in what is going to affect the rest of my life. There is no more free education. Especially when your parents are making you pay for it by yourself like I am. At least I have a very good, well-paying job, In N Out. In N Out has really taught me how to be responsible with my money. It has made me realize how fast your money can disappear. It has also broken me out of my shell. In N Out has taught me how to deal with angry customers and how to talk to anyone. These are some very important traits I feel that I will need for the rest of my life. Step 2: Evaluate your Current Job A. What are the tasks that you are performing in your current job that will transfer to future jobs and your career? * The tasks that I am performing in my current job that will help me transfer to future jobs and my career is dealing with agitated people, working at a fast pace, being able to talk to anyone, and being able to do what people tell me quickly and efficiently. Dealing with angry and/or agitated customers is probably the hardest part of the job. Not only is it awkward, but it is also very agitating for you. I normally want to just scream in their face that it’s not my fault but I always keep my cool. Working at a fast pace without stressing is also a very good skill to have. You have to be able to work very fast at In N Out. We are constantly busy and having to please hundreds of customers a day. B. What are the possibilities for advancement at this current job, and what are the skills you could still learn at this job? * In N Out runs off of a level system. A level 1 cleans the dining room. A level 2 takes orders and can work the back pay window. A level 3 can take orders outside with something we call a hand held, and can also hand out food for the drive through. A level 4 works fries. A level 5 dresses, puts the condiments, on the burger. A level 6 actually cooks the burgers. Lastly, a level 7 is a manager’s assistant. They help run the shift when the manager isn’t around. Then after all those are the managers. Each separate store has 4 different mangers. They move up as well. They all start out as 4th managers and then move up to a 1st manager, which is a â€Å"store manager†. After that they can move up to district managers and then a regional manager. I am currently a level 3 but I am hopefully getting my level 4 soon. I’ve been working and learning fries. In N Out is definitely my fall back if the ultrasound technician fails. Step 3: Interests A. What do you like to do? * I love to hang out with friends, my boyfriend, go out to eat, and sleep. I can hang out with my friend and Jason for days. Probably like every other teenager but that’s when I have the most fun. Going out to eat is my favorite thing to do. I don’t really care if it’s bad for you. I love food. Sleeping is what I do in my free time. I am constantly tired so I love sleep when I actually have the time to do so. B. What is the best experience you have ever had? The best experience I’ve ever had was probably my senior prom. Basically it was the best night of my life. It was amazing. From getting ready, to pictures, to dinner, to the bus, to the dance, to the bus again, and then Kyle Wades spa. I got to get ready with my best friends and my boyfriend’s mom paid for me to get my makeup done. I love that woman. The pictures were great with m y boyfriend and best friends. We got some really cute ones. Dinner was delicious at chilis. The bus was the best part in addition to the actual dance. We had a stripper pole and all the guys got on it. This is the irst dance I’ve been to that the teachers didn’t care how we danced. By the time we got off the dance floor everyone was dripping sweat. My legs were sore for 3 days after. After that we hopped back on the bus, made a quick stop to In N Out and then chilled in Kyle’s spa. Jason and I had to sleep in my truck that night but we woke up early and went to Denny’s for breakfast. C. What makes you the happiest? * My boyfriend and friends are definitely my top priority at this point. They have made me who I am today. D. What makes you feel most satisfied? * Making everyone around me happy and accomplishing a new goal. E. When have you felt the most rewarded? * When I reached my level 3 and got my raise because I earned it all on my own with no one else’s help. Step 4: Aptitudes A. What are you good at? * I am very good at following orders. When someone tells me to do something I will get it done right away and get it done very well. B. 1. After the first set of questions, what are the top 10 careers that come up? * 1. Choreographer / Dance Instructor * 2. Correctional Officer * 3. Director of Photography * 4. Director * 5. Athletic Trainer * 6. Security Guard * 7. Vending Machine Servicer * 8. Kinesiologist * 9. Auto Detailer 10. Stock Clerk 2. How do you feel about these careers? I don’t this a lot of these careers suit me at all. 3. What are the results now, how did they change? 1. Bailiff 2. Artist 3. Special Effects Technician 4. Computer Network Specialist 5. Stuntperson 6. Director of Photography 7. Cardiologist 8. Doctor 9. Anesthesiologist 10. Nurse Practitioner 4. Did your t op 3 interests appear? What are they and why do they interest you? My top 3 interest groups did not appear at all. Ultrasound technician, Crime Scene investigator, or working in advertisement. They all seem like jobs you can’t get bored of.